Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta

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Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta1

PREMISE OF THE STUDY Here we present a series of protocols for RNA extraction across a diverse array of plants; we focus on woody, aromatic, aquatic, and other chemically complex taxa. METHODS AND RESULTS Ninety-one taxa were subjected to RNA extraction with three methods presented here: (1) TRIzol/TURBO DNA-free kits using the manufacturer's protocol with the addition of sarkosyl; (2) a comb...

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ژورنال

عنوان ژورنال: Applications in Plant Sciences

سال: 2015

ISSN: 2168-0450,2168-0450

DOI: 10.3732/apps.1400105